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KMID : 1005420130150030171
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Journal of Cerebrovascular and Endovascular Neurosurgery
2013 Volume.15 No. 3 p.171 ~ p.176
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The Role of Adiponectin in Secondary Inflammatory Reaction in Cerebral Ischemia
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Jung Yong-Su
Ha Sung-Kon
Kim Sang-Dae
Kim Se-Hoon
Lim Dong-Jun
Choi Jong-Il
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Abstract
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Objective: In this study, we investigate the role of adiponectin in the interaction between leukocytes and endothelium in the secondary inflammatory reaction of cerebral ischemia
Methods: Adiponectin knock-out mice group (APN-KO) (n = 8) and wild-type mice group (WT) (n = 8) were prepared. Each group was sub-divided into 2 groups by reperfusion time. One-hour middle cerebral artery occlusion and reperfusion were induced using the intraluminal filament technique. At 6 and 12 hours after the occlusion, the mice were placed on a stereotactic frame to perform craniotomy in the left parietal area. After craniotomy, a straight pial venule was selected as a target vessel. With the fluorescence intravital microscope, the number of rolling leukocytes and leukocytes that adhered to endothelium were counted and documented at 6 and 12 hours after the reperfusion.
Results: At 6 and 12 hours after the reperfusion, more rolling leukocyte and leukocyte adhesion were observed in the APN-KO mice than in the WT mice. The difference in leukocyte numbers between the APN-KO and WT mice was found to be statistically significant (p = 0.029) by Mann-Whitney U-test.
Conclusion: We found that adiponectin inhibits the interaction between the endothelium and leukocytes in cerebral ischemia-reperfusion. Therefore adiponectin might prevent the secondary insult caused by the inflammation reaction.
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KEYWORD
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Adiponectin, Cerebral ischemia, Secondary inflammatory reaction, MCAO-R
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